Measurement

 principles

Precision-engineered motorized scanning Nanoparticle Tracking Analysis (NTA) instrument for tracking the movement of individual visualized nanoparticles in suspension

Real-time visualization of Brownian Motion and electrophoretic mobility, for measuring size, concentration and zeta potential in scattering and fluorescence modes.

Four simultaneous aligned and software-controlled lasers for fluorescence measurements

Software controlled emission filter changer for quick changes between the fluorescence measurements

Fast scanning to acquire and analyze typically 1000 particles in ~ 1 minute

Software-controlled pumps for flushing and sample sub-dosing

 Samples

Nanoparticles suspended in polar liquids (e.g. water, alcohols) for size, concentration, fluorescence and zeta potential studies

 Optical layout

90° laser scattering video microscope with x10 magnification

Automated alignment and focusing of laser and microscope"

 Camera

High sensitive CMOS camera 640 x 480 pixels

Variable frame rate from 1 to 60 Hz for optimum resolution and fast acquisition"

 Fluorescence

Software controlled, automated filter wheel with four long-pass fluorescence emission filters(LWP) with cut-off at 430 nm / 500 nm / 550 nm / 680 nm [QUATT]

Bandpass filter available on request

 Cell Assembly

Z-NTA slide-in assembly for size, concentration and dual fluorescence measurements plus zeta potential experiments in aqueous and organic solvents with pumps for 2 different liquids/buffers – for rinsing and sub-dosing experiments, electrical field sensing

 Cleaning

Cell cleaning recommended weekly – cell resistant to >1000 brush cleanings

Cleaning of driver electrodes required after more than 1000 zeta potential runs

Cleaning kit and basic replacement parts included in delivery

 Temperature

Working external temperature range: 5°C ~ 45°C

Sample temperature control : Peltier temperature control from RTP -5°C ~ 55°C with dew-point sensing

 Communication

Software provided on pre-configured PC, communication via Ethernet

 Quality Control

Cell quality check, daily performance check, outlier control with automatic Grubbs statistical analysis of measurement data

 Live Monitoring

Fully customizable SOPs for different samples / applications

 Standard

 Operating

Cell quality check, daily performance check, outlier control with automatic Grubbs statistical analysis of measurement data

 Analysis and

 Reports

Data Analysis in scatter and / or fluorescence mode: particle size distribution profiles, concentration, overlays and averaging, scatter plots, zeta-potential distribution profiles, sub-population analysis

Data export format: AVI, TXT, CSV, FCS

PDF reports containing key results

 Size/Concentration

Concentration range: 105 ~ 109 particles/ml

Particle size: 10nm ~ 2000nm (dependent on sample and laser selection)

Accuracy: ±5nm (for 100nm polystyrene latex)

Reproducibility: ±2nm (for 100nm polystyrene latex)

 Fluorescence

Concentration range: 105 ~ 109 particles/ml

Particle size: 10nm ~ 2000nm (dependent on fluorescent dye and laser selection)

Accuracy: ±5nm (for 100nm polystyrene latex)

Reproducibility: ±2nm (for 100nm polystyrene latex)

 Zeta Potential

Working range: -500mV ~ +500mV

Concentration range: 106 ~1010 particles/ml

Conductivity range: 3µS/cm ~ 15mS/cm

Accuracy: ±4mV (for alumina zeta potential standard)

Reproducibility: ±2mV (for alumina zeta-potential standard)

 General

Minimum sample quantity: 500μl of sample at 10 particles/ml

pH range: 1 ~ 13

Temperature: 5°C ~ 45°C (external temperature)

Sample volume visualised and tracked by the camera for a single measurement: 11 x 3nL

 Reference

Nominal 100 nm reference suspension for size

Two nominal 100 nm reference suspensions for fluorescence

Nominal +50mV reference suspension for zeta potential

 Physical

Footprint (W x D x H) : 20 x 30 x 25cm

Weight : 8.5kg (main unit, PC extra)

Shipping box with standard content : 48 x 62 x 63cm ; 22kg

 Electrical

90~240V, 47~63Hz, 50VA